An inexpensive substrate for the production of alkaline protease by Bacillus sp and its application studies of
نویسنده
چکیده
Protease executes a large variety of functions and it has many biotechnological applications. It represents as one of the third largest group of industrial enzyme and finds its applications in detergents, leather industry, pharmaceutical industry etc. Among the different vegetable waste products, peel of Manihot esculenta (Tapioca/ cassava) was used as substrates for the production of protease by Bacillus sp. The aim of our study is to utilize the waste of Tapioca peel as input for protease production using Bacillus sp. The protease produced by Bacillus sp from M. esculenta showed a pH of 10 and temperature of about 30°C. Protease produced was tested for possible industrial applications. This enzyme shows high capacity in removing the blood stain, dehairing the animal skin, digestion of natural proteins and bioprocessing of used X-ray film. The wash performance analysis of blood stains on cotton fabrics showed that blood stains were completely removed within 15 minutes of incubation of fabrics along with enzyme. Complete hair removal of goat skin by the protease achieved within 12 hours of incubation at 30°C. This enzyme has the ability to dissolve the blood clot and coagulated egg within 20 minutes of incubation. Enymatic hydrolysis of gelatin from waste X-ray films was also investigated. At the end of the treatment, gelatin layer was completely removed leaving the polyester film clean. Gelatin hydrolysis was monitored by measuring increase in turbidity. Gelatin layer was removed completely within 10 minutes of addition of enzyme. The protease produced exhibits high antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The present study was a humble attempt towards the production of protease and to check their efficiency in industrial applications. These properties indicate the possibilities of enzyme usage in various industries and it can be exploited commercially in future.
منابع مشابه
Response of surface optimization for the enhanced production of alkaline protease isolated from Bacillus sp. with bean husk as a new substrate
Optimization of the fermentation medium for maximum alkaline protease production was carried out. Fifteen positive isolates were examined for their extent of alkaline protease production. The most potent producer was identified as Bacillus sp. The solid substrate screening showed that the combination of wheat straw and bean husk was the best one. The initial screening by using Plackett–Burman’s...
متن کاملBatch Kinetics and Modeling of Alkaline Protease Production by Isolated Bacillus sp. (RESEARCH NOTE)
The aim of this study was the use of fish waste hydrolysate (FWH) as a substrate for alkaline protease production using isolated Bacillus sp. in a batch system. Then the fermentation kinetics of enzyme production was studied. The results show that with the addition of FWH to the fermentation medium with a final concentration of 4% (optimal concentration), alkaline protease value reached a maxim...
متن کاملStudy on Activity and Stability of Proteases from Bacillus Sp. Produced by Submerged Fermentation
Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...
متن کاملStudy on Activity and Stability of Proteases from Bacillus Sp. Produced by Submerged Fermentation
Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...
متن کاملOptimization of alkaline protease production from a locally isolated Bacillus sp. ZR-5: Potential application as a detergent additive
The aim of this study was to optimize protease production using a novel Bacillus sp. ZR-5 strainisolated from the soil, and evaluate its application in detergent industry. The proteolytic activityof the strain was demonstrated using gelatin hydrolysis screening test. Protease productionoptimization was carried out using a two-step approach: a conventional method in order toidentify the best car...
متن کاملCloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis
in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
متن کامل